Abstract:
Morphological peculiarities of liver after antigen influence on the mother-placenta-fetus system is not well studied. Materials and methods: The study included the livers of 144 white laboratory rats from 1 to 90 days of life. All animals were conditionally divided into 3 groups: Group I - intact animals, which were born from healthy rats without any antigen administration during pregnancy; group II – control animals, which were exposed to antenatal intrafetal administration of 0.05 ml saline solution on the 18th day of prenatal development; group III – experimental animals, which were exposed to antenatal antigen administration at 18th day of prenatal development. The absolute and relative mass of the liver was calculated. For light microscopy, sections were stained with hematoxylin and eosin. Detection of β-D-galactose carbohydrate residues was performed using a Lectin from Ricinus communis Agglutinin (RCA) by standard methods, using standard set of reagents "LectinTest" (Lviv). Quantitative and qualitative morphological characteristics of the liver were studied. Analysis of the obtained results was conducted by means of statistical methods with the use of computer license program “Statistica for Windows 13” (StatSoft Inc., № JPZ804I382130ARCN10-J). Results: In experimental animals after antenatal administration of staphylococcal toxoid, the relative area occupied by hepatic plates (as in the control) tends to increase gradually during the first two weeks of life. In animals, which were exposed to antenatal Staphylococcal purified liquid toxoid administration, the number of lymphocytes in the central zone of the liver lobule is lower than in the intact and control groups on the first day is 1.04 ± 0.35 cells on unit area. (2.26 ± 0.52 cells on unit area in control). The maximum decrease in the number of lymphocytes in the central zone in experimental rats in contrast to the control occurs on the 30th day of life together with a simultaneous decrease in the number of Kupffer macrophages and is 0.70 ± 0.35 cells on unit area – in the experiment (2.61 ± 0.69 cells on unit area in the control group). A decrease in the number of lymphocytes is observed also in the peripheral zone of the lobule compared with the control from the third to the 30th day of life, but such changes have no statistical significance. It was found that RCA+ receptors are expressed on dendritic cells in the liver, the number of RCA+ dendritic cells changes in waves during the first three months after birth. Conclusions: The relative mass of the liver in experimental group rats significantly exceeds the control at the seventh day of life (3.06 ± 0.05% in the experimental and 2.91 ± 0.02% in the control group). In experimental rats the number of dendritic cells decreases during the first two weeks of life, the minimum number is observed on the 3rd day. In the peripheral zone of the lobule, a decrease in the number of dendritic cells is observed on the 1st and the 21st day of postnatal life in comparison with control.
