Abstract:
Objective: The aim of this work is the synthesis and studying of antimicrobial and antifungal properties
of the ylidenhydrazides of 2-((4-R-5-R1-4H-1,2,4-triazol-3-yl)thio)acetaldehydes with the establishment of
structure-activity relationships of the synthesized compounds.
Material and Method: Determination of antimicrobial and antifungal activity was performed by discdiffusion method (DDM) on series of gram positive and gram negative bacteria such as Corynebacterium
pseudodiphtheriticum, Pseudomonas aeruginosa, Enterococcus faecalis, Proteus vulgaris, Escherichia coli,
Salmonella spp., Staphylococcus saprophyticus, Staphylococcus aureus, Streptococcus pyogenes, Candida.
Result and Discussion: A new series of ylidenhydrazides of 2-((4-R-5-R1-4H-1,2,4-triazol-3-
yl)thio)acetaldehydes were synthesized and their structures were confirmed by modern methods of
instrumental analysis: IR and 1H-spectroscopy, elemental analysis and GS\MS method. For 13 compounds,
the antimicrobial and antifungal activities were studied on 10 strains of microorganisms in 3 different
concentrations (0.1; 0.2; 0.5%). According to the results of the study, some regularities of the structureactivity relationship have been established. The obtained results can serve as a basis for the targeted search
compounds with pronounced antibacterial action. An increasing the concentration of tested compounds
predictably lead to increase on antimicrobial activity in almost all cases. The most active compound in our
study is N'-(2-((5-methyl-4H-1,2,4-triazol-3-yl)thio)ethylidene)-4-nitrobenzohydrazide 3i (ZOI for Sta.s. and
Sp in 0.5% concentration is 25 and 26 mm, respectively).
