Abstract:
Introduction. The emergence of multiple drug resistance in bacteria has become one of the most terrible challenges of this century: the prevalence of infections that are difficult to treat is increasing, and there are no appropriate therapeutic alternatives. Therefore, the development and implementation of biomaterials in the form of bioactive or therapeutic wound dressings with integrated bioactive molecules (antiseptics) for achieving controlled antibacterial treatment is relevant and promising.
Aim. To investigate the antimicrobial activity of new biomaterials developed on the basis of decamethoxine, polyvinyl alcohol and calcium alginate, and modern commercially available antimicrobial wound dressings against reference and clinical strains of causative agents of healthcare-associated wound infections, K.pneumoniae, A .baumannii and P.aeruginosa. Object and methods. Antimicrobial properties of new biomaterials with decamethoxine (DCM №1-3) and commercially available wound dressings Suprasorb® X + PHMB, SILVERCEL® Hydro-Alginate, Urgotul SSD®, GUANPOLISEPT®, Bétadine TULLE 10% DRESSING were studied on reference and clinical polyresistant strains of K.pneumoniae, A. baumannii, P.aeruginosa by the disk diffusion method (Kirby-Bauer test) with registration and comparison of the diameters of zones of inhibition (ZOI). Results. Comparative studies of antimicrobial properties of developed biomaterials and commercially available antimicrobial dressings revealed high antimicrobial properties of new decamethoxin-based biomaterials against reerence and clinical strains of target microorganisms, which are leading pathogens of wounds and burns. Reference and clinical strains of A.baumannii show the greatest susceptibility to samples with decamethoxin (№ 1-3 DCM), as well as to biomaterials Suprasorb® and Guanpolisept® based on polyhexanide. Biomaterials with decamethoxin №1, №2 and №3, Suprasorb®, Guanpolisept® and Bétadine® were determined to be the most effective against reference and clinical strains of K.pneumoniae. Reference and clinical strains of P.aeruginosa are most susceptible to biomaterials with decamethoxin №1, №2 and №3 and Bétadine®.
Conclusions. The developed biomaterials were not inferior to modern effective wound dressings based on cationic detergents and silver-containing wound dressings, they actively inhibited the growth of reference and clinical strains of K.pneumoniae, A.baumannii, P.aeruginosa, and were often the most active. The effectiveness of silvercontaining wound dressings in vitro was inferior to the effectiveness of dressings with cationic detergents and iodophors.
