Abstract:
Sirtuin 1 (SIRT1) emerges as a promising biomarker for heart remodeling in thecontext of essential arterial hypertension (EAH). Additionally, the levels of certain plasma peptidesmight signal myocardial fibrosis and the progression of heart failure (HF) in hypertensive patients.Despite this potential, conflicting data exist regarding the marker's diagnostic value in HF patients.This study aims to explore the impact of anthropometric factors on plasma SIRT1 levels in men di-agnosed with EAH and HF, considering the rs7069102 single nucleotide polymorphism (SNP) in theSIRT1 gene.Methods: The study included an examination of 190 Ukrainian men aged between40 and 65. The participants were divided into two groups: a control group, consisting of 70 indi-viduals without cardiovascular disease (CVD), and a study group comprising 120 men with EAH,60 of whom displayed signs of HF. Plasma SIRT1 levels were quantified using an enzyme-linked im-munosorbentassay(ELISA),whilethers7069102C/GpolymorphismintheSIRT1genewasdetectedthrough allele-specific polymerase chain reaction (PCR). The research employed various statisticalmethods, including correlation analysis, T-test, Mann-Whitney U test, one-way ANOVA, and analysisof contingency tables for data analysis.Results: The investigation revealed that men suffering fromEAH and HF exhibited significantly reduced plasma SIRT1 levels (1.550 0.084 ng/ml) comparedto those with EAH but without HF (3.271 0.238 ng/ml, p < 0.001). Notably, hypertensive menwith concurrent HF and obesity or those with an early onset of hypertension showed even lowerplasma SIRT1 concentrations. Interestingly, the analysis found no significant difference in plasmaSIRT1 levels among individuals in the control group and EAH patients without HF across differentrs7069102 C/G SNP variants. However, among hypertensive men with HF, individuals with the GGgenotype displayed considerably lower plasma peptide levels compared to those with either theCC or CG genotype (1.390 0.092 ng/mlvs. 1.744 0.126 ng/ml, p = 0.032).Conclusion: Thisstudy highlights a significant association between reduced plasma SIRT1 levels and heart failurein male patients with EAH. Factors contributing to decreased plasma peptide levels include obe-sity, early onset of hypertension, and possessing the GG variant of the rs7069102 SNP in the SIRT1gene. These findings underscore the potential of SIRT1 as a marker for HF and may guide futuretherapeutic strategies.
