Abstract:
Introduction. Opportunistic pathogens, which normally vegetate on the conjunctival mucosa, are involved in the development of perioperative infectious complications in ophthalmic practice. When examining the contents of the conjunctival sac of healthy individuals by the culture technique, coagulase- negative staphylococci, which are always present on the eyelid skin, are most often isolated in small quantities. Bacteria of this genus belong to opportunistic pathogens, since with mechanical, chemical or thermal injury to the skin or mucous membranes, they are actively engaged in the development of an inflammatory reaction.Aim. The purpose of the work was to determine the prophylactic and therapeutic efficacy of antimicrobial agents widely used in ophthalmology by studying the sensitivity of coagulase- negative staphylococci isolated from the conjunctival contents to the mentioned agents.Materials and methods. Bacteriological examination of the conjunctival content of 20 healthy individuals and 10 individuals with signs of post-traumatic conjunctivitis was carried out, 17 strains of S. epidermidiswere isolated.Results. There were no significant differences in the level of susceptibility to the studied agents of S. epidermidis strains isolated from the surface of a healthy conjunctiva compared to strains isolated from the injured mucosa. Both were highly resistant to the most long-used drugs Sulfacil sodium and levomycetin.The mean MIC of tobramycin for isolates obtained from intact conjunctiva amounted to 8.3±3.4 μg/ml and was significantly lower than that of the antiseptic miramistin (20.8±4.2 μg/ml). The obtained indices of the antistaphylococcal activity of decametoxin suggest a high prophylactic and therapeutic activity of the eye drops. After all, the concentration of the antiseptic in eye drops accounts for 200 μg/ml, which is 20 times more than the highest determined MBC for coagulase- negative staphylococci.Conclusions. The study results of the sensitivity of the obtained isolates to antimicrobial agents showed a low level of efficacy of Sulfacil sodium and levomycetin, doubtful sensitivity to levofloxacin and miramistin and a high level of antistaphylococcal activity of decametoxin and tobramycin
