Abstract:
Aim. To evaluate the effect of mesenchymal stem cells of various origins, rat embryonic fibroblasts and Wharton’s jelly MSCs lysate on the dynamics of destructive changes in the somatosensory cortex of rats with artificially induced acute cerebrovascular injury.
Materials and methods. Modelling of transient bilateral ischemia-reperfusion (IR) in rats by ligation of the internal carotid arteries. Transplantation of mesenchymal stem cells (MSCs) of different origins into the femoral vein of experimental animals. Cytological study on 7th and 14th days after IR with counting the total number of neuronal nuclei in 1 mm2 in the somatosensory cortex of animals and the ratio of the intact neuronal nuclei to the pathologically altered nuclei.
Results. Transplantation of MSCs, rat embryonic fibroblasts, MSC lysate from human umbilical cord Wharton’s jelly (hUC-WJ) and administration of citicoline led to an increase in the number of intact neurons and nuclei without pathological changes in the somatosensory cortex of rats. The most effective in terms ofKeywords: ischemia-reperfusion; hippocampus; neuroapoptotic changes; flow cytometry; mesenchymal stromalcells cytomorphology was the transplantation of hUC-WJ MSCs.
Conclusions. The MSC transplantation is effective in the treatment of cerebral ischemia. The most effective prevention of destructive changes was observed in the somatosensory cortex of rats that were transplanted with MSCs from human umbilical cord Wharton’s jelly after IR.
