Abstract:
The technical difficulties of serial intravesical administration of drugs are a significantproblem in preclinical studies of the effectiveness of local chemotherapy for superficialbladder cancer. The aim of the study was to develop and evaluate the effectiveness ofthe method of serial intravesical administration of drugs in the experiment. The studyincluded 49 Wistar rats of both sexes. Five proposed methods of intravesicaladministration of drugs were investigated: retrograde catheterization of the urinarybladder under anesthesia, method of transcutaneous puncture of the urinary bladder inrats, intraoperative puncture of the bladder, method of subcutaneous fixation of thebladder for subsequent puncture in the experiment, method of serial intravesicaladministration of drugs in female rats using special catheter. The obtained data wereprocessed using a package of statistical programs SPSS 20.0 for Windows. The disadvantage of the first technique is its trauma - each insertion of the catheter isaccompanied by trauma of the urethral mucosa and associated pain. The manipulationswere failed because of edema of the urethral mucosa. The manipulation should beperformed under general anesthesia. The second technique also requires generalanesthesia. The aggressive cytostatic action of Doxorubicin prevented the closure ofthe puncture hole and caused the development of complications. Sealing the bladderwith a collagen plate when performing the third technique prevents the release of thedrug into the abdominal cavity, however, the adhesion of the tissues of the surroundingorgans to the plate promotes the formation of an adhesive conglomerate and makesfurther manipulations impossible. The withdrawal of the bladder from the abdominalcavity during the implementation of the fourth technique, theoretically, should havehelped to avoid the complications observed during the third technique. However,insufficiently reliable fixation of the urinary and adhesions in the area of the implantedring make this technique such that it does not correspond to the tasks set. The fifthmethod was the only one proposed that met all the assigned tasks. This techniquemade it possible to perform a series of 5 intravesical injections of Doxorubicin withoutgeneral anesthesia and without the development of postoperative complications fromthe postoperative wound and abdominal cavity. The proposed method of serialintravesical administration of drugs in female rats using a special catheter makes ittechnically easy to perform serial intravesical administration of drugs without the use ofgeneral anesthesia and without the development of complications from the postoperativewound and abdominal cavity.
