Immunohistochemical analysis of the inducible and endothelial fractions of NO-synthase in the intenstinal mucosa of colo-colonic anastomosis under influence of chronic stress and thiotriazoline application

Abstract

UUnder stress in the development of metabolic disorders, nitric oxide (NO) occupies a significant place. Dependence of the metabolism of NO indicates its important role in the pathogenesis of diseases of the gastrointestinal tract. The aim of the study is to study immunohistochemical changes in the colon after the large bowel resection and the effect of stress and correction of thiatriazoline. The experiment was performed on 86 male rats. After considering the results of our examination, a long-term exposure to stress of the experimental animals leads to a sig nificant increase of the inducible expression of NO - synthase fractions (iNOs) in the cells of the mucous membrane of the large bowel in the zone of anastomosis. Under the conditions of chronic immobilization stress, the use of tiotriazolini enhances the expression of the constitutive endothelial isoform of NO - synthase in the endothelium of blood vessels in comparison with the animals, which were exposed to stress and underwent resection without drug correction. The intraperitoneal injection of tiotriazolini better regulates stress-mediated changes in the expression of inducible and endothelial isoforms of NO - synthase and normalizes the content of nitrogen oxide in the large bowel of the rats during the final stages, while subserous injectin is more effective in the early stages of examination, that reflects the compensation of the endothelial dysfunction and the reduction of the effect of nitrogen oxide.