Study of antimicrobial effect of Сhrysanthemum×hortorum bailey pectoral variety flowers and leaves

Abstract

The aim. The aim of our study was to determine the antimicrobial effect of 10% and 20% aqueous solutions of Chrysanthemum x hortorum Bailey flowers and leaves Pectoral variery. Materials and methods. 10 % and 20 % native aqueous solutions of flowers and leaves of Chrysanthemum x hortorum Bailey Pectoral variery were studied. The antibacterial action of the samples was determined regarding 9 test cultures: gram-positive microorganisms Staphylococcus aureus ATCC 6538, Staphylococcus epidermidis ATCC 14990, Micrococcus luteus NCTC 2665, Corunebacterium spp ATCC 373, Serratia marcescens, gram-negative microorganisms Klebsiella pneumoniae ATCC13883, Escherichia coli ATCC 25922, spore culture Bacillus cеrеus NCTC 74. The antifungal effect was studied against Candida albicans ATCC 855/653. The bacteriostatic effect of the studied samples was determined by the results of growth of reference strains in native aqueous solution of the corresponding samples and in dilutions of 1:1, 1:2 in meat-peptone broth; bactericidal action – in the absence of growth of the contents of test tubes with native solutions of the samples and in a 1:1 dilution in dense nutrient media. The following media were used to determine the bactericidal effect: meat-peptone agar for all cocci and bacilli, blood sugar agar – for Klebsiella and corynebacteria, Endo medium – for enterobacteria, Saburo medium – for yeast-like fungi of the Candida genus. Results. It was established that the native 10% aqueous solution of Chrysanthemum x hortorum Bailey Pectoral variery flowers had a bactericidal effect against Staphylococcus epidermidis and Microcoсcus luteus, delayed growth and reproduction (bacteriostatic effect) of Staphylococcus aureus and Escherichia coli and and did not affect the growth of Klebsiella, corynebacteria, sera, and Candida fungi. Dilution of 10% solution of chrysanthemum flowers 1:1 had a bacteriostatic effect only on the test culture of staphylococci. Antibacterial effect of 10% aqueous solution of Chrysanthemum x hortorum Bailey Pectoral variery leaves was active only in the native form, it was not manifested when diluted. 20% aqueous solution of flowers and leaves of Chrysanthemum x hortorum Bailey Pectoral variery proved to have antimicrobial activity similar to the previous 10% solutions. Conclusions. It has been established that all the studied samples of flowers and leaves of chrysanthemums have antimicrobial activity. Native 10 % and 20 % aqueous solutions of chrysanthemum flowers and leaves has shown antibacterial activity against gram-positive and gram-negative microflora, so they are promising for development of a drug with antimicrobial properties.