Study of the Stress-Protective Effect of Sodium 2-((4-amino-5-(thiophen-2-ylmethyl)- 4H-1,2,4-triazol-3-yl)thio)acetate

Abstract

The aim was to study the stress-protective effect of sodium 2-((4-amino-5- (thiophen-2-ylmethyl)-4H-1,2,4-triazol-3-yl)thio)acetate (ASP). The analysis of the effect of the sample on the behavior and psycho-emotional state of animals, physiological parameters, antioxidant-prooxidant system was studied. Studies of stress-protective properties were performed on white outbred rats. As a compari son drug was used a Mebicar (Adaptol®) (reference sample). One hour after the simulation of immobilization 6-hour stress, the animals were subjected to a series of tests: “Open Field”, “Light and Dark Chamber”, “Morris Water Labyrinth”. Behavioral tests were performed according to generally ac cepted methods in simplified modifications. Serum and liver homogenate were used to determine the content of markers of the antioxidant-prooxidant system: quantitative content of diene conjugates (DC), thiobarbituric acid reactants (TBA reactants), as well as catalase and superoxide dismutase (SOD) activity. Based on complex behavioral tests data, Catalase, TBA reactants, DC, SOD data in the serum and in the liver homogenate it can be concluded the presence of stress-protective properties of ASP in the model of acute immobilization stress.