The Relationship between Serum IGF-1 and Hydroxyproline Levels in the Rats with Chronic Toxic Hepatitis

Abstract

Abstract. The values of profibrogenic cytokine IGF-1 content in the rats under conditions of experimentally modeled chronic toxic hepatitis and drug correction with lizinopril are presented in the article. Experimental studies were carried out on 60 non-linear white laboratory immature rats, with an initial body weight of 50-70 g. The animals were divided into 5 groups - 12 rats in each group. Group 1 consisted of intact rats. Chronic toxic hepatitis was modeled in the animals of groups 2, 3, 4 and 5 by intragastric administration of 20% ССl4 Solutio oleosa in the dose of 0.1 ml/100 g of body weight and 5% ethanol solution twice a week [7]. The rats of group 2 were administered no other agents. Along with hepatotoxins, the animals of the remaining groups received angiotensin-converting enzyme inhibitor (ACE inhibitor) “Lizinopril” (“Astrapharm”, Ukrain) in the dose of 20 mg/kg/day (group 3), intragastric “Glutargin” (0.75g, LLC "Zdorovya") in therapeutic and prophylactic dose of 30 mg/kg/day (group 4) [14], intragastric 20% ССl4 Solutio oleosa in the dose of 0.1 ml/100 g of body weight twice a week in combination with 5% ethanol solution, as well as “Glutargin” (0.75g, LLC "Zdorovya") in the dose of 30 mg/kg and “Lizinopril” in the dose of 20 mg/kg (20 mg, LLC “Astrapharm”) (group 5). The use of lizinopril in experimental CTH resulted in normalization of serum IGF-1 level, which is likely to have favorable prognosis in chronic liver pathology. Besides, strong direct correlation relationship between IGF and PHP concentration was found in the animals who received corrective combination therapy with lizinopril and glutargin, indicated by significant decrease of PHP on the background of IGF concentration decrease.