Functional and morphological state of the amputation stump muscles after various methods of the stump plasty

Abstract

An assessment of the nature of histochemical and morphological processes in limb stump muscles after various methods of the amputation plasty was given. There were five series of experiments carried out on 55 dogs with the amputation of the hind limb in the middle third of the thigh. There was fascioplasty, myoplasty with suture of the antagonist muscles performed, as well as myodesis with the muscle tension of 916-962 μN, 980-1100 μN, and 650-800 μN. The observation periods consisted of 3 days, 4 weeks, 8 months. The histochemical reactions for determining of succinate dehydrogenase (SDG), NAD•N-dehydrogenase (NAD•H-DH), beta-hydroxybutyrate dehydrogenase (β-HBDG) and lactate dehydrogenase (LDG) were performed on the fresh muscle pieces. The histological examination of the muscles was also carried out. By the end of the observation period, only single unchanged muscular fibers were detected by fascio- and myoplasty. Most of them were replaced by the coarse fibrous connective tissue with fat cells. There was a significant increase in LDG activity (glycolysis) and growth of SDG (Krebs cycle). The activity of NAD•H-DH was high, and the level of β-HBDG significantly reduced. By myodesis with the muscle tension of 916-962 μN the degenerative-dystrophic processes were less significant. There was the decrease in the intensity of the terminal oxidation and oxidation of the ketone bodies observed. The activity of the Krebs cycle increased, and glycolysis grew up. By muscles tension of 980-1100 μN and 650-800 μN there were changes similar to those by fascio- and myoplasty observed.