The effect of ademol on the dna fragmentation of cerebral cortex cells in rats with experimental traumatic brain injury

Abstract

The aim of the study. To determine the effect of Ademol on the deoxyribonucleic acid (DNA) fragmentation of the cerebral cortex cells (apoptosis) of rats with traumatic brain injury (TBI). Materials and methods. An experimental model of severe trauma was created in male rats using an air pistol. The therapeutic effect of Ademol in TBI was evaluated at a dose of 2 mg/kg intravenously at intervals of 2 t/d for 8 days. As a drug for the control group we used 0.9% NaCl at a dose of 2 ml/kg, and as a comparison drug - amantadine sulfate at a dose of 5 mg/kg. On day 8 after TBI and decapitation of animals, the parts of the 146 cerebral cortex were taken to assess further DNA fragmentation in cells by the flow cytometry method. Results and conclusions. The post-traumatic period of model TBI in rats is accompanied by a probable increase in the level of DNA fragmentation in the nucleus of cerebral cortex cells on the 8th day of the experiment. By the antiapoptotic effect in conditions of post-traumatic brain injury, Ademol solution therapy was significantly better than the infusion of 0.9% NaCl and amantadine sulfate at an average of 46.2 and 27.2%, respectively (p<0.05)