Histological study of a corrective influence of sodium 2-((4-amino-5-(thiophen-2-ylmethyl)-4h-1,2,4-triazol-3-yl) acetate on the state of rats liver under conditions of acute immobilization stress

Abstract

Objective: The aim of the work was the histological study of a corrective influence of sodium 2-((4- amino-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-3-yl) thio) acetate on the state of rats liver under conditions of acute immobilization stress. Material and Method: As an object of research sodium 2-((4-amino-5-(thiophen-2-ylmethyl)-4H-1,2,4- triazol-3-yl) thio) acetate was used. All substances were injected intragastrically, daily for 5 days in appropriate doses on an empty stomach from 9.00-10.00. 1 hour after the last injection, immobilization 6-hour stress was simulated. The central part of the liver was fixed for further histological examination in 10% formaldehyde solution, dehydrated in alcohols of increasing strength, poured into paraffin. Result and Discussion: The results of the study demonstrate the presence of stress-protective properties of sodium 2-((4-amino-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-3-yl) thio) acetate in the model of acute immobilization stress, which was reproduced for 6 hours. As can be seen from the described and presented microscopic picture, the test sample to some extent reduced the degree of stress damage to the liver. In terms of the positive effect on the histological condition of the liver parenchyma (a more pronounced structural orientation of hepatocytes was noted, their beam structure was contoured, dystrophic changes of hepatocytes are less pronounced, round cell infiltrates and foci of hepatocyte necrosis are absent), sodium 2-((4-amino-5- (thiophen-2-ylmethyl)-4H-1,2,4-triazol-3-yl)thio)acetate exhibits a stress-protective effect in the model of acute immobilization stress. We can conclude about similarity of activity of the test sample with comparison drug (Mebicar®) at studying of influence on a histologic condition of a liver parenchyma