Gene polymorphism of ЕT–1 and its plasma levels in men with uncomplicated essential hypertension and left ventricular hypertrophy

Abstract

Aim: To improve diagnosis of the severity of essential hypertension in men citizens of Podillia region in Ukraine by determining the plasma levels of ET-1 in patients with different ET-1 gene variants. Methods. We examined 141 men aged 40 – 60 years old, who live in Podillia region in Ukraine. Among them 62 men were diagnosed uncomplicated EH with left ventricular hypertrophy and CHF I classe according to NYHA Classification. 79 healthy men were included into a control group. The patients with uncomplicated EH and the healthy men were representative by age. The ET-1 (Lys198Asn) gene polymorphism was determined by PCR, and the level of ET-1 plasma concentrations was established by ELISA. Results. In both healthy men and patients with uncomplicated EH with LVH, residents of Podillia region in Ukraine aged 40-60 years old, dominates Lys198Lys genotype and Lys allele of the ET-1 gene. It was found that any inherited variant of the ET-1 gene was not associated with the risk of developing uncomplicated EH with LVH in men residents of Podillia region in Ukraine. However, the carriers of the Asn allele of the ET-1 gene have significantly higher levels of ET-1 in plasma in both healthy men and patients with uncomplicated EH and LVH. There are calculated levels of ET-1 that can be used for screening of large groups of people for early diagnosis of uncomplicated EH with LVH in carriers of the Asn allele and carriers of the genotype Lys198Lys of the ET-1 gene.