STUDY OF CARBONIC ACIDS OF ROOTS COMARUM PALUSTRE L.

Abstract

Actuality. According to the literature, the underground organs of Comarum palustre L. contain organic acids, and there is no information about their component composition. The purpose of the study is to study the component composition of organic acids in the roots of Comarum palustre L. and determine the quantitative content of the sum of organic acids in 7 series of raw materials present on the pharmaceutical market of Ukraine. Material and methods. Plant raw materials for research were purchased in the spring of 2024 in online markets of Ukraine specializing in the sale of medicinal plant raw materials: series 1 – “Chlorophytum”, series 2 – “Sednivski travy”, series 3 – “Svit trav”, series 4 – TM “Organic herbs”, series 5 – “PbayMarket”, series 6 – “Narod pharma”, series 7 – “Dary Karpat”. The component composition of organic acids was studied by gas chromatography-mass spectrometry. The quantitative content of the sum of organic acids was determined by the method of the monograph of the State Pharmacopoeia of Ukraine 2.1 “Rosehips FruitsN”. Research results. In the roots of Comarum palustre, for the first time, the presence of 6 dicarboxylic acids was established by the method of chromatography-mass spectrometry and the quantitative content of each of them was determined. Their total content was 1462.49 μg\g. Three carboxylic acids were dominant in content: levulinic (almost 47% of the total content of identified acids), citric and malic acids (28.49% and 15.41%, respectively). For the first time, in 7 batches of raw materials purchased in Ukraine, the quantitative content of the sum of organic acids in terms of malic acid was determined in the roots of Comarum palustre, using the modified method of the monograph of the State Pharmacopoeia of Ukraine 2.1 “Rosehips FruitsN”. The quantitative content of organic acids varies more than 1.2 times – from 1.98% (series 1) to 2.41% (series 6). The validation characteristics of the method were established for the first time and tested on five batches of raw materials. Conclusions. For the first time, the component composition of organic acids was established in the roots of Comarum palustre by the method of gas chromatography-mass spectrometry. For the first time, the content of organic acids in 7 series of Comarum palustre roots purchased in specialized marketplaces of Ukraine was determined. The obtained data will be used in further pharmacognostic research of Comarum palustre roots.