Короткий опис (реферат):
Actuality. Polyphenolic compounds exhibit significant antioxidant, anti-inflammatory, and immunomodulatory properties. These compounds actively participate in various biochemical processes in plants and substantially enhance the biological effects of numerous medicinal plants and the pharmaceutical products derived from them. The cultivated species of marigolds spreading (Tagetes erecta L.), along with its forms and varieties, serves as a valuable source of carotenoids for medical and economic applications. During the growing season, its inflorescences accumulate polyphenolic compounds characterized by pronounced biological activity. However, the specific composition and quantitative content of these compounds under the environmental conditions of southeastern Ukraine throughout the growing season remain unexplored. Aim of study is determination of the component composition and quantitative content of polyphenolic compounds in the inflorescences of Tagetes erecta L. var. “Antigua F 1 Orange”. Material and methods. The plant material consisted of air-dried inflorescences of Tagetes erecta L. cultivated in Ukraine, variety var. “Antigua F1 Orange”, collected during the flowering period (June – September 2023–2024). The component composition and quantitative content of polyphenolic compounds were determined using thin-layer chromatography (TLC) on glass-backed plates “Merkieselguhr F254” 20 x 20 (Merck KGaA, Germany) with the densitometer device “Biostep” CD 60 (Germany) and high-performance liquid chromatography (HPLC) “Shimadzu LC-20” with a UV detector (Japan). Standard compound samples were sourced from “Supelco Analytical, Sigma-Aldrich” (USA). Research results. In the inflorescences of Tagetes erecta L. var. “Antigua F1 Orange”, the quantitative composition of 22 primary polyphenolic compounds was identified and analyzed. Among these, 13 were classified as flavonoids, while 9 were categorized as hydroxycinnamic acids. The quantitative flavonoid contents were 20,40 ± 2,14 and hydroxycinnamic acids 5,60 ± 0,64% respectively. Conclusion. The substantial quantitative content of flavonoids and hydroxycinnamic acids in the inflorescences of Tagetes erecta L. var. “Antigua F1 Orange” is of significant importance for the standardization of plant raw materials, preparations derived from them, and their subsequent incorporation into medical practice.
